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KMID : 0923620080080010029
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Immune Network
2008 Volume.8 No. 1 p.29 ~ p.36
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IL-23 P19 Expression Induced by IL-17 and IL-1β in Rheumatoid Arthritis Synovial Mononuclear Cells
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Cho Mi-La
Heo Yu-Jung
Oh Hye-Jwa
Kang Chang-Min
Lee Seon-Yeong
Hong Yeon-Sik
Kim Ho-Youn
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Abstract
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Interleukin-23 (IL-23) is a novel pro-inflammatory cytokine which has been implicated to play a pathogenic role in rheumatoid arthritis (RA). This study was undertaken to investigate the IL-23 inductive activity of the proinflammatory cytokine IL-17, IL-1? and tumor necrosis factor (TNF-?) in RA synovial fluid mononuclear cells (SFMC). Expression of IL-23p19, IL-17, IL-1? and TNF-? in joint was examined by immunohistochemistry (IHC) of patients with RA and osteoarthritis (OA). The effects of IL-17 and IL-1? on expression of IL-23p19 in human SFMC from RA patients were determined by reverse transcriptase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). IL-23p19 was expressed in the RA fibroblast like synoviocyte (FLS), but not from OA FLS. Similar to the protein expression, IL-23p19 mRNA could be detected by RT-PCR in RA SFMC. IL-17 and IL-1? could induce RA SFMC to produce the IL-23p19. The effects of IL-17 were much stronger than IL-1? or TNF-?. These responses were observed in a dose- responsive manner. In addition, IL-17 or IL-1? neutralizing antibody down-regulated the expression of IL-23p19 induced by LPS in RA-SFMC. Our results demonstrate that IL-23p19 is overexpressed in RA synovium and IL-17 and IL-1? appears to upregulate the expression of IL-23p19 in RA-SFMC. [Immune Network 2008;8(1):29-37]
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KEYWORD
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Rheumatoid arthritis, Synovial fluid mononuclear cells, IL-23p19, IL-17, IL-1β
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